FG0465

IMDM

With stable-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• 别名: Cell culture medium, Iscove′s MDM, Iscove’s Modified Dulbecco’s Medium, Modified Dulbecco′s medium
• 分類程式碼代碼: 12352207
• NACRES: NA.71

属性

• product name: Iscove′s Modified Dulbecco′s Medium, With L-alanyl-L-glutamine and sodium bicarbonate, sterile-filtered, liquid, suitable for cell culture
• 品質等級: 400
• 無菌: sterile-filtered; sterile
• 產品線: AQmedia^™
• 形狀: liquid
• 技術: cell culture | mammalian: suitable
• 雜質: Endotoxin, tested
• 成分: stable glutamine: yes; NaHCO₃: yes; glucose: yes; phenol red: yes
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

说明

一般說明

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

應用

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco¢s Modified Eagle¢s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove¢s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

法律資訊

AQmedia is a trademark of Sigma-Aldrich Co. LLC

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable