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Merck

D5540

Sigma-Aldrich

心肌黄酶 来源于克鲁氏梭状芽孢

lyophilized powder, 3.0-20.0 units/mg protein (biuret)

别名:

心肌黄酶, 硫辛酰胺脱氢酶, 硫辛酰脱氢酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Clostridium kluyveri)

品質等級

形狀

lyophilized powder

比活性

3.0-20.0 units/mg protein (biuret)

運輸包裝

wet ice

儲存溫度

−20°C

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應用

含亚甲蓝(MB)的聚丙烯酰胺纳米粒子平台(NPs)已在含有明胶酶(来源于克氏梭菌)和辅酶NADH的溶液进行了测试。这项试验的目的是检验将MB封装于纳米粒子中是否能阻止MB的还原,从而保护其光动力效应。来自Sigma的酶与醛脱氢酶可一起通过固定化用于构建乙醛生物传感器。它也被用于将NADP再氧化成NADPH。该反应可同时催化刃天青转化为高荧光的试卤灵,并且还可实现对微量NAADP的检测。NAADP最初会被其他酶转化为NADP和NADPH。
来自克氏梭菌的心肌黄酶,或称为硫辛酰脱氢酶,已被用于评估在有氧代谢过程中,硫辛酸在2-氧代酸脱氢酶上进行组装时的蛋白质-蛋白质相互作用。在一项研究中,硫辛酰脱氢酶也被用于研究酪氨酸硝化的氧化还原调节和抗氧化剂对3-硝基酪氨酸的还原。

包裝

以天然心肌黄酶单位进行出售。

其他說明

“心肌黄酶”这一名称已粗略地应用于几种酶,这些酶在电子受体如亚甲蓝或2,6-二氯苯酚吲哚的存在下,可催化β-NADH或β-NADPH的氧化。这些酶以不同的“单位”以应用于许多不同的检测中。
这些酶是已知对β-NADH或β-NADPH特异的心肌细胞酶。Straub的猪心脏酶可能具有天然心肌黄酶(β-NADH特异性)以及硫辛酸和硫辛酰胺脱氢酶活性。据报道它是一种单体蛋白质。然而,Massey报道“心肌黄酶”可能是一种变性的脂硫辛酰胺脱氢酶。将猪心脏制剂与Cu2+进行预孵育,可降低硫辛酰胺脱氢酶活性并呈比例增加β-NADH心肌黄酶活性。在我们的实验室中,我们已经在一定程度上证明了这种对猪心脏酶的铜效应,但在克氏梭菌 或圆酵母制剂中没有观察到明显的效果。硫辛酰胺脱氢酶:心肌黄酶的比率可作为一种变性的量度。

單位定義

在pH7.5,25℃条件下,一个单位的“心肌黄酶”或“硫辛酰”脱氢酶每分钟可氧化1.0μmole的β-NADH,同时还原相应的2,6-二氯苯酚吲哚

抑制劑

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Wei Tang et al.
Biochemical and biophysical research communications, 369(2), 579-583 (2008-02-27)
The ability to prevent methylene blue (MB), a photosensitizer, from being reduced by plasma reductases will greatly improve its efficacy in photodynamic therapy (PDT) applications. We have developed a delivery approach for PDT by encapsulating MB using nanoparticle platforms (NPs).
Bachar H Hassan et al.
The Journal of biological chemistry, 286(10), 8263-8276 (2011-01-07)
Lipoic acid is a covalently attached cofactor essential for the activity of 2-oxoacid dehydrogenases and the glycine cleavage system. In the absence of lipoic acid modification, the dehydrogenases are inactive, and aerobic metabolism is blocked. In Escherichia coli, two pathways
Andreas Gasser et al.
The Journal of biological chemistry, 281(25), 16906-16913 (2006-04-22)
Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent activator of Ca2+ release from intracellular stores known today. Although recent reports have suggested an important function of NAADP in human T lymphocytes, direct evidence for receptor-induced formation of NAADP
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Chembiochem : a European journal of chemical biology, 9(2), 312-323 (2007-12-29)
Covalent modifications of proteins by endogenous reactive nitrogen oxide species lead to cytotoxic effects that are implicated in diseases associated with chronic infections and inflammation. Tyrosine nitration is a major post-translational modification of proteins by reactive nitrogen oxide species. Recent
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