A373-C6

97011321, human skin, Epithelial-like

• 分類程式碼代碼: 41106514

属性

• 产品名称: A373-C6, 97011321
• 生物源: human skin
• 包裝: tube of 5 μg 97011321-DNA-5UG; pkg of vial of cells 97011321-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: IL-1 receptor
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human malignant melanoma IL-1 sensitive

細胞系描述

The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (Sigma Catalogue number. 88113005) by limiting dilution together with its twin clone A375-C5 (Sigma Catalogue number. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.

應用

IL-1 growth inhibition assay, IL-1 receptor analysis

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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