A375-C5 Cell Line human

97011320, human skin, Epithelial-like

• 分類程式碼代碼: 41106514

属性

• product name: A375-C5 Cell Line human, 97011320
• 生物源: human skin
• 描述: Human malignant melanoma IL-1 insensitive
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human malignant melanoma IL-1 insensitive

細胞系描述

The cell line A375-C5 is unresponsive to the anti-proliferative effect of IL-1. It has been derived from the parental line A373 (Sigma catalogue no. 88113005) which was subjected to cloning by limiting dilution. This method resulted in the establishment of A375-C5 and an IL-1 sensitive clone, A375-C6 (Sigma catalogue no. 97011321). The unresponsiveness of A375-C5 is probably due to an IL-1 receptor deficiency. The cells have been eradicated from mycoplasma at ECACC.

應用

IL-1 resistance studies, receptor studies

培養基

RPMI 1640 + 2mM Glutamine + 10% FBS

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

其他說明

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