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product name
NCI-H69/LX10, 96042331
生物源
human lung
增長模式
Aggregates in suspension
染色體組型
Not specified
形態學
Not specified
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human small cell lung cancer, drug-resistant
細胞系描述
The drug-resistant cell line NCI-H69/LX10 has been derived from the parent line NCI-H69 (ECACC No 91091802). It is recommended to culture the cells without drug upon resuscitation; add doxorubicine once growth is fully established.
應用
Drug-resistance studies
培養基
RPMI 1640 + 2mM Glutamine + 1.0 μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2, 37ºC; resuscitate in drug-free medium and add drug after growth has been established.
其他說明
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