RIN-5F

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 95090402, rat pancreas, Not specified

• 分類程式碼代碼: 41106514

属性

• 生物源: rat pancreas
• 包裝: tube of 5 μg 95090402-DNA-5UG; pkg of vial of cells 95090402-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Insulin, L-dopa-decarboxylase
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat islet cell tumour

細胞系描述

RIN-5F is a secondary clone of the rat islet tumour cell line RIN-m (Sigma Catalogue number. 95071701). Cloning was performed by limited dilution in RPMI 1640, 10%FBS and 20% conditioned medium. The conditioned medium contained growth hormones and prolactin being produced by rat pituitary tumour cells GH3 (Sigma Catalogue number. 87012603). RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, L-dopa-decarboxylase and contain membrane-bound secretory granules.

應用

Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow in small islands and do not form a confluent monolayer.

其他說明

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