BC9

Reactivity: Anti-TNF, bovine, 95030301

• 分類程式碼代碼: 41106514

属性

• 生物源: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: P3X63Ag8.653
• 反應: Anti-TNF, bovine
• 技術: cell culture | mammalian: suitable
• 同型: IgG1
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse P3X63Ag8.653 x Mouse BALB/c spleen

細胞系描述

The monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.

免疫原

Bovine recombinant TNF-alpha

應用

Detection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).

培養基

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 5-6x100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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