C2

94101906, rat liver, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: C2, 94101906
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: Hyperdiploid, 52 Chromosomes
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat hepatoma [HGPRT-]

細胞系描述

Full name Faof1C2 is a dedifferentiated variant of Fao. These cells continue to express tyrosine aminotransferase, but the liver-specific markers are mostly absent.

應用

Gene expression analysis.

培養基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures 1:50 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 10x10,000,000,000 cells/10cm² dish.

其他說明

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