FGC4

94101904, rat skin, Epithelial

• 分類程式碼代碼: 41106514

属性

• 产品名称: FGC4, 94101904
• 生物源: rat skin
• 增長模式: Adherent
• 染色體組型: Modal no. 52
• 形態學: Epithelial
• 產品: Albumin and ß-fibrogen
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat hepatoma [alb-gpt plasmid]

細胞系描述

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

應用

Gene expression studies.

培養基

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

其他說明

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