KYSE-70

94072012, human esophagus, Epithelial

• 别名: KY70 Cells, KYSE70 Cells
• 分類程式碼代碼: 41106514

属性

• product name: KYSE-70, 94072012
• 生物源: human esophagus
• 增長模式: Adherent
• 染色體組型: Hypotriploid with 8% polyploidy
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human oesophageal squamous cell carcinoma

細胞系描述

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

其他說明

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