TGP 49

94022427

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse pancreas
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Trabecular
• 產品: Somatostatin (low levels)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse pancreatic acinar carcinoma

細胞系描述

TGP 49 has been isolated from an acinar cell carcinoma arising in the pancreas of a transgenic Tg(E1a-1-SV40E)Bri18 mouse. These mice carry a pseudogene construct composed of a pancreatic specific elastase-1 promoter linked to the SV40T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 49 secretes low amounts of somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. They have been reported not to secrete amylase or lipase but do secrete trypsin. Differentiation characteristics fluctuate from time to time in culture. Trabecular and clumps of small cells.

應用

Study of endocrine pancreas carcinomas, hormone assays.

培養基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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