DSL6A/C1

94022431, rat pancreas, Polygonal

• 分類程式碼代碼: 41106514

属性

• 产品名称: DSL6A/C1, 94022431
• 生物源: rat pancreas
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Polygonal
• 產品: Expresses CFTR protein
• 受體: Lacks CCK-A receptors
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat pancreatic carcinoma

細胞系描述

DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.

應用

Study of pancreatic carcinomas, differentiation of acinar/ductal cells.

培養基

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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