KELLY Cell Line human

92110411, human brain, Neuronal

• 别名: NB19 Cells, NB19-RIKEN Cells
• 分類程式碼代碼: 41106514

属性

• 产品名称: KELLY Cell Line human, 92110411, neuroblastoma
• 生物源: human brain
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: N-myc RNA or protein
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human neuroblastoma

細胞系描述

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

應用

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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