TM3

91060526, mouse testis, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: TM3, 91060526
• 生物源: mouse testis
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Prostaglandin F2a
• 受體: Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c testis, Leydig cell

細胞系描述

Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.

應用

Hormone studies

培養基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37²C.

其他說明

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