C2C12 Cell Line from mouse

91031101, mouse muscle, Myoblast

• 别名: C2-C12 Cells
• 分類程式碼代碼: 41106514

属性

• product name: C2C12 Cell Line from mouse, C3H muscle myoblast, 91031101
• 生物源: mouse muscle
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Myoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C3H muscle myoblast

細胞系描述

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

應用

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

培養基

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

例行更新培養

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.