EB-3

90121003

• 分類程式碼代碼: 41106514

属性

• 生物源: human unknown/unspecified
• 增長模式: Suspension
• 染色體組型: 2n = 46
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Burkitt′s lymphoma

細胞系描述

The cell line EB3 has been established in 1964 from a 3 year old male with Burkitt′s lymphoma. Herpes-like virus particles have been detected by electron microscopy. Antibodies from patients with infectious mononucleosis react with the herpes type virus (EBV) found in EB3 cells. The cell line has been shown to be resistant to challenge with vesicular stomatitis virus. Lymphoblastoid, grow as single cells and in clumps. Ethnicity: Black.

應用

Study of Burkitt′s lymphoma, virus sensitivity

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12,15
D13S317: 12,14
D16S539: 10,12
D5S818: 9,10
D7S820: 11
THO1: 7
TPOX: 6,9
vWA: 17,19

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO₂; 37°C

其他說明

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