Hep2 (HeLa derivative) Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86030501, human cervix, Epithelial

• 分類程式碼代碼: 41106514

属性

• 生物源: human cervix
• 描述: Human Black, cervix carcinoma
• 包裝: tube of 5 μg 86030501-DNA-5UG; pkg of vial of cells 86030501-1VL
• 增長模式: Adherent
• 染色體組型: Modal no. 14
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human cervix carcinoma

細胞系描述

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

應用

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. After thawing the first subculture may take 7-10 days.

其他說明

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