Clone C6/36

89051705, insect larvae, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: Clone C6/36, 89051705
• 生物源: insect larvae
• 增長模式: Adherent
• 染色體組型: Diploid
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mosquito larvae, Aedes albopictus

細胞系描述

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

應用

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO₃) level of 1.1g/l is recommended).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm² . Cells are dislodged by mechanical agitation; 5% CO₂; 28°C. Freeze in 10% glycerol.

其他說明

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