Bu (IMR-31)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

属性

• 生物源: buffalo lung
• 包裝: tube of 5 μg 89051704-DNA-5UG; pkg of vial of cells 89051704-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 60
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Buffalo lung

細胞系描述

Derived from female Buffalo yearling. The cells have a finite life span of a minimum of 30 passages and are susceptible to a range of viruses including HSV, vaccinia, PRV, BVDV, para-influenza 3, bovine enterovirus (M62/4); also IBRV and VSV (Indiana). The cell line produces both detectable BVDV antigens and infectious BVDV.

應用

Virus replication studies

培養基

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.