FM3Ats C1.T85

87111904, mouse breast, Lymphoblast

• 分類程式碼代碼: 41106514
• 生物源: mouse breast
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 相關疾病: cancer

属性

• 产品名称: FM3Ats C1.T85, 87111904
• 生物源: mouse breast
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C3H mammary carcinoma

細胞系描述

A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (Sigma Catalogue number. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.

應用

Tumourigenicity studies, chromosome condensation studies.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 1-3x100,000 cells/ml; 5% CO₂; 33°C.

其他說明

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