COLO 205 Cell Line human

87061208

• 别名: COLO205 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human colon
• 描述: Human Caucasian colon adenocarcinoma
• 增長模式: Suspension
• 染色體組型: Hypertriploid modal no 71-78
• 形態學: Round and refractile cells in suspension. Some cuboidal cells in the monolayer
• 產品: Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

一般說明

Isolated from ascitic fluid of a 70 year old Caucasian male with carcinoma of the colon. Produces carcinoembryonic antigen.

細胞系來源

Human Caucasian colon adenocarcinoma

細胞系描述

COLO 205, COLO 201 (Sigma product no. 87091201) and COLO 206 (Sigma product no. 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 205 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 205 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer.

應用

tumourigenic in suitable animals

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split subconfluent cultures of attached cells 1:3 to 1:6 seeding at 2-3x10⁴ cells/cm²; 5% CO₂; 37°C.

其他說明

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