LoVo Cell Line human

87060101, from human colon(supraclavicular lymph node metastasis)

• 别名: LoVo Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human colon (supraclavicular lymph node metastasis)
• 增長模式: Adherent
• 染色體組型: Modal no. 49, (2n=46)
• 形態學: Epithelial
• 產品: Carcinoembryonic antigen (CEA)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human colon adenocarcinoma

細胞系描述

Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA).

應用

Human LoVo cell line is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to test the in vitro cytotoxic property and anti-proliferative property of C. lechleri stem bark essential oil.

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18

培養基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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