IMR 32

86041809

• 别名: IMR32 Cells, Institute for Medical Research-32 Cells
• 分類程式碼代碼: 41106514

属性

• 生物源: human nerve
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Fibroblast/neuroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian neuroblastoma

細胞系描述

Established from an abdominal mass occurring in a 13 month old Caucasian male. The tumour was diagnosed as a neuroblastoma with two rare areas of orgonoid differentiation. The culture is a mixture of two morphologically distinct cell types, a small neuroblast-like cell and the other a large hyaline fibroblast. IMR 32 is partially resistant to polioviruses type 3 and highly resistant to ECHO-II. It is susceptible to Coxsackie B3.

應用

IMR 32 has been used to study the transmigration of cells through a human in vitro blood-cerebrospinal fluid barrier (BCSFB) model.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The cells have a low plating efficiency and cell attachment can take up to 2-4 days after resuscitation. Cells easily detach. May n

其他說明

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