ES-E14TG2a

08021401, mouse embryo (blastocyst). Monolayer of spheroidal cells on feeder layer of PMEFs or STOs

• 别名: E14TG2a Cell Line
• 分類程式碼代碼: 41106514

属性

• product name: ES-E14TG2a, 08021401, from mouse embryo(blastocyst)
• 生物源: mouse embryo (blastocyst)
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

说明

細胞系來源

Mouse (strain 129/Ola) embryonic stem cell

細胞系描述

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

DNA分析

Not specified

培養基

DMEM + 2 mM Glutamine + 1% NEAA + 0.1 mM 2-Mercaptoethanol + 1000 U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Fetal Bovine Serum. Renewal of medium every 2-3 days is required.

例行更新培養

Not specified

其他說明

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