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一般說明
胶原是体内广泛表达的蛋白。人体组织中有20种不同类型的胶原蛋白。I型胶原是丰富的骨蛋白。它构成了约90%的骨有机物。
使用Bornstein和Traub命名的胶原蛋白术语源自参考文献;Bornstein, P. and Traub, W. The Proteins, (1979) 4, 411-605
胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用Bornstein和Traub提出的命名法。不要混淆Sigma分类标识和公认的胶原蛋白分类类型。
應用
来自牛跟腱的胶原适合用于:
- 胶原酶活性的检测
- 作为使用差示扫描量热法,热重法,气相色谱法和傅里叶变换红外光谱法进行人体骨骼热分析研究中的参考样品
- 作为开发用于确定体外胶原降解的简单测定的底物
- 固相酶联免疫吸附试验检测整合糖蛋白二肽基肽酶IV与不溶性I型胶原结合活性的研究
牛跟腱胶原蛋白用于植物提取物的研究中,作为MMP-胶原酶的抑制剂,MMP-胶原酶是骨关节炎和类风湿关节炎中软骨分解的推定活性剂。
生化/生理作用
来自牛跟腱的胶原是天然存在的蛋白,呈细长的原纤维形式。它可用于纤维软骨区的研究,胶原蛋白在插入跟骨之前必须首先穿过纤维软骨区。它也可用于研究生长因子对跟腱愈合过程中胶原含量和交联的影响。
胶原是一种不溶性纤维蛋白,是细胞外基质和结缔组织的一部分。它负责组织承受拉伸的能力。 称为前胶原的长前体在ER中合成和组装,分泌到细胞外空间并加工形成胶原纤维。不同类型的胶原蛋白由包含三个多肽链的分子组成,这些多肽链以三重螺旋构象排列,氨基酸序列略有不同。一级结构是在每三个位置具有甘氨酸的重复基序,经常在脯氨酸或4-羟脯氨酸残基之前。
準備報告
根据Einbinder, J. and Schubert, M., J. Biol. Chem., 188, 335 (1951)中的方法制备。
重構
本品为不溶性胶原制剂。它不溶于水,水性缓冲液,稀酸和有机溶剂。为了在胶原酶测定中用作底物,该胶原可以制备为在含0.36 mM氯化钙的50mM TES缓冲液(pH值7.4)中的混悬液。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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