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Merck

C7902

Sigma-Aldrich

氯化钙 二水合物

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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About This Item

经验公式(希尔记法):
CaCl2 · 2H2O
CAS号:
分子量:
147.01
EC號碼:
MDL號碼:
分類程式碼代碼:
12352302
PubChem物質ID:
NACRES:
NA.71

等級

reagent

產品線

BioReagent

化驗

≥99.0%

形狀

powder

技術

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

溶解度

H2O: 294 mg/mL, clear, colorless

SMILES 字串

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

InChI 密鑰

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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一般說明

氯化钙(CaCl2)是一种常用的生化试剂。它在信号转导、肌肉收缩、维持细胞膜和细胞壁稳定等生物学过程中发挥着重要作用。CaCl2 用于大肠杆菌E. Coli)的制备和转化,以及真核细胞的质粒)的制备和转化,以及真核细胞与质粒 DNA 或高分子量基因组 DNA 的转染。已有研究显示质粒 DNA pBR322 可通过 CaCl2 介导的电穿孔法导入大肠杆菌。CaCl2 用于大肠杆菌 NADH-泛醌氧化还原酶(络合物 I)的稳定和二维结晶。在 CaCl2 和柠檬酸钠环境下,猪胰腺弹性蛋白酶的结晶揭示了蛋白质金属结合位点的钙结合。CaCl2大肠杆菌微管蛋白样丝状温度敏感突变体 Z(FtsZ)分裂蛋白聚合过程中总荧光强度的影响已通过多孔荧光检测法进行了研究。

應用

二水合氯化钙已用于:
  • 培养基制剂
  • 精子收集和制备
  • 细胞培养和原生质体制备

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

实验方案

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry

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