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重組細胞
expressed in E. coli
品質等級
化驗
>90% (SDS-PAGE)
形狀
lyophilized powder
比活性
13,000 units/mg protein
分子量
~30 kDa
溶解度
PBS: soluble
運輸包裝
dry ice
儲存溫度
−70°C
生化／生理作用
Cleaves the substrates consisting of consensus sequence VEID.
Spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme.
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays.
單位定義
One unit will hydrolyze 1 nmol of the caspase substrate VEID-pNA to VEID and p-nitroaniline per hour at pH 7.2 at 37 °C.
外觀
Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.
Ana A Baburamani et al.
Developmental neuroscience, 37(4-5), 321-337 (2015-04-01)
Apoptotic mechanisms are centre stage for the development of injury in the immature brain, and caspases have been shown to play a pivotal role during brain development and in response to injury. The inhibition of caspases using broad-spectrum agents such
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