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螢光
λex 496 nm; λem 516 nm
運輸包裝
wet ice
儲存溫度
2-8°C
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一般說明
Baseclick EdU In Vivo Kit为全部动物的细胞增殖检测提供了替代BrdU 及[[3H]thymidine检测的出色方法，且未显示出任何的毒性效果。EdU（5-乙炔基-2'.-脱氧尿苷）是一种胸苷核苷类似物，在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同，EdU In Vivo Assay不依赖抗体，因而不需进行DNA变性来检测掺入的核苷。恰恰相反，EdU In Vivo Kit利用点击化学法来检测不同种类的染料荧光示值。此外，该试剂盒的实验方案经过简化，减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成，在结果的内容和情境丰富程度方面，堪比多重分析法。这一款体内试剂盒的设计秉承一种理念，即使您可以结合如下三种baseclick EdU细胞增殖试剂盒之中的一种：
1. 成像试剂盒 (BCK-IV-IM)
2. 流式细胞术试剂盒(BCK-IV-FC)
3. 高通量筛选 – HTS试剂盒 (BCK-IV-HTS)
您可以针对自己的动物模型选择上述试剂盒及自选的染料和合适的EdU含量。
根据您的动物模型或需要检测的动物数量，您可以选择S、M和L三种规格的试剂盒，这三种规格的EdU含量依次递增，如下表所示。
试剂盒规格 EdU含量
S 50 mg
M 500 mg
L 1000 mg
1. 成像试剂盒 (BCK-IV-IM)
2. 流式细胞术试剂盒(BCK-IV-FC)
3. 高通量筛选 – HTS试剂盒 (BCK-IV-HTS)
您可以针对自己的动物模型选择上述试剂盒及自选的染料和合适的EdU含量。
根据您的动物模型或需要检测的动物数量，您可以选择S、M和L三种规格的试剂盒，这三种规格的EdU含量依次递增，如下表所示。
试剂盒规格 EdU含量
S 50 mg
M 500 mg
L 1000 mg
應用
体内，成像
訊號詞
Danger
危險分類
Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 1B - Repr. 2
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
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Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
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