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Merck

B9183

Sigma-Aldrich

单克隆抗-HA

clone HA-7, purified from hybridoma cell culture

别名:

抗-HA, 抗-流感血凝素

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.56

生物源

mouse

共軛

biotin conjugate

抗體表格

purified from hybridoma cell culture

抗體產品種類

primary antibodies

無性繁殖

HA-7, monoclonal

形狀

buffered aqueous solution

濃度

~1 mg/mL

技術

western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.

運輸包裝

dry ice

儲存溫度

−20°C
2-8°C

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一般說明

The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.

特異性

该抗体可识别HA-标签蛋白的天然和还原变性形式,并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。

免疫原

与人流感病毒血凝素(HA)片段相对应的合成肽,称为HA-标签,与KLH结合

應用

Antibody suitable for immunoblotting.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells
Tragoolpua,K et al.
Biotechnology, 8(5) (2008)
J Zhang et al.
Scientific reports, 8(1), 2373-2373 (2018-02-07)
High-risk, cancer-causing human papillomaviruses (HPV) cause infections of the epidermis that may progress to cancer, including cervical cancer. Viral persistence, contributed to by viral evasion of the host immune response, is associated with the likelihood of cancer developing. Langerhans cells
Emeline Ragonnaud et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 40(2), 51-61 (2017-02-07)
Currently available prophylactic vaccines have no therapeutic efficacy for preexisting human papillomavirus (HPVs) infections, do not target all oncogenic HPVs and are insufficient to eliminate the burden of HPV induced cancer. We aim to develop an alternative HPV vaccine which
Antonio Galeone et al.
eLife, 6 (2017-08-23)
Mutations in the human

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