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product name
Amphiregulin human, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)
生物源
human
品質等級
重組細胞
expressed in E. coli
化驗
≥97% (SDS-PAGE)
形狀
lyophilized powder
效力
10-100 ng/mL ED50/EC50
分子量
~11 kDa
包裝
pkg of 1 mg
儲存條件
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
UniProt登錄號
儲存溫度
−20°C
基因資訊
human ... AREG(374)
相关类别
生化／生理作用
Also known as keratinocyte autocrine factor (KAF); expression of amphiregulin mRNA has been found in numerous carcinoma cell lines and epithelial cells of several human tissues including colon, stomach, breast, ovary, and kidney.
成分
Human amphiregulin is produced by a DNA sequence that encodes a 98 amino acid residue form of mature human amphiregulin. This corresponds to amino acid residues 101-198 in E. coli. Amphiregulin is a glycoprotein produced in response to treatment of the cell line MCF-7, a human breast carcinoma cell line, with PMA, a protein kinase C activator and potent tumor promoter.
注意
This product should be stored at -20°C. After preparation, the product can be stored at 2-8°C for one month, or frozen in aliquots at -20°C or -70°C for more extended storage.
外觀
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4 with 50 μg BSA per 1 μg as a carrier protein.
準備報告
It should be reconstituted by adding sterile PBS containing 0.1% HAS or BSA to a concentration greater than 10 μg/mL.
分析報告
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. et al. (1984) Science 223:1079. The ED50 for this effect is typically 5-15 ng/mL.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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