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生物源
bovine spleen
品質等級
種類
Type IX
形狀
ammonium sulfate suspension
比活性
150-200 units/mg protein
分子量
32.5-33 kDa
UniProt登錄號
異物活動
5′-AMP deaminase ≤0.01%
alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%
儲存溫度
2-8°C
基因資訊
cow ... ADA(280712)
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一般說明
分子量:32.5-33 kDa
pI: 4.85
pI: 4.85
腺苷脱氨酶/ ADA是一种酶,主要存在于淋巴细胞和单核细胞中。
應用
来自牛脾脏的腺苷脱氨酶已被用于代谢三磷酸腺苷 (ATP) 和腺苷至无活性形式(肌苷)。它也被用作 KRH(Krebs-用 Hepes 缓冲的林格氏液(含 CaCl2)培养基,以便除去用于环磷酸腺苷 (cAMP) 测定的外源性腺苷。
腺苷脱氨酶可用于各种分子生物学检测,如甘油释放试验等。腺苷脱氨酶是治疗合并免疫缺陷疾病的潜在靶标。
生化/生理作用
腺苷脱氨酶/ ADA可用作鉴定结核性胸腔积液的重要标志。 ADA的活性通常在肝病结核病、伤寒、感染性单核细胞增多症和一些恶性肿瘤中较高。
腺苷脱氨酶是嘌呤分解代谢酶,其以近似相等的特异性催化腺苷和2'-脱氧腺苷的脱氨基。它普遍存在于哺乳动物组织中,腺苷脱氨酶缺乏会导致严重的联合免疫缺陷病。
單位定義
在25°C的pH 7.5下,每分钟一个单位将1.0μmol的腺苷脱氨为肌苷。
外觀
3.2M (NH4)2SO4,0.01M 磷酸钾溶液中的悬浮液,pH 6.0
分析報告
由缩二脲测定的蛋白质。
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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