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生物源
avidin from egg white
enzyme from horseradish
品質等級
共軛
peroxidase conjugate
形狀
lyophilized powder
成份
Protein, ≥70% E1%/280
標籤範圍
0.7-2.0 mol peroxidase per mol avidin
技術
direct ELISA: 1:50,000
溶解度
H2O: soluble 1 mg/mL
PBS: soluble
儲存溫度
−20°C
一般說明
亲和素是来自于蛋清的同源四聚体蛋白(68kDa)。这种蛋蛋白质与生物素高度结合。因此,亲和素生物素结合已被用于免疫检测,以检测组织中的抗原定位。亲和素-生物素免疫检测的使用增强了该技术的灵敏度并改善了对低含量抗原的检测。
應用
为了检测小鼠血清中的Ga1缺陷型IgA1抗体,可在96孔板中使用血清进行ELISA检测,其中,血清与生物素化HAA(结合IgA1)一起孵育。然后将HRP偶联的亲和素与血清在37℃孵育40分钟。
亲和素-过氧化物酶已被用于检测抗5-溴脱氧尿苷(抗BrdU)结合和OVA-IgG1。
外觀
含有柠檬酸盐缓冲液的冻干粉末。
準備報告
Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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