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Merck

A2556

Sigma-Aldrich

Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse

clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution

别名:

Monoclonal Anti-Rabbit IgG (γ-chain specific)

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

共軛

alkaline phosphatase conjugate

抗體表格

purified immunoglobulin

抗體產品種類

secondary antibodies

無性繁殖

RG-96, monoclonal

形狀

buffered aqueous glycerol solution

物種活性

rabbit

應無反應活性

goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken

技術

direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells

同型

IgG1

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

Monoclonal Anti-Rabbit IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

應用

Monoclonal Anti-Rabbit IgG (γ-chain specific)Alkaline Phosphatase antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

生化/生理作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

外觀

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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The Laboratory Rat (1998)
The Immunoglobulins: Structure and Function (1998)
Regulation of the megakaryocytic glycoprotein IX promoter by the oncogenic Ets transcription factor Fli-1
Bastian LS, et al.
Blood, 93(8), 2637-2644 (1999)
Factors determining the density of AQP4 water channel molecules at the brain-blood interface
Hoddevik EH, et al.
Brain Structure &Amp; Function, 222(4), 1753-1766 (2017)
Serena Varotto et al.
Plant physiology, 133(2), 606-617 (2003-09-16)
We analyzed the expression profile and cellular localization of the maize (Zea mays) Rpd3-type histone deacetylases genes ZmRpd3/101, ZmRpd3/102, and ZmRpd3/108 (indicated as ZmHDA101, ZmHDA102, and ZmHDA108 in the Plant Chromatin Database). This study shows that maize Rpd3 transcripts are

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