A211140

DMT-2′O-Methyl-rA(bz) 磷酰胺

configured for MerMade

• 别名: DMT-2′-O-甲基-rA(bz)酰胺, N-苯甲酰-5′-O- [双（4-甲氧苯基）苯甲基]-2′-O-甲基-腺苷，3′-[2-氰乙基 N,N-双（1-甲基乙基）亚磷酰胺]
• 经验公式（希尔记法）: C₄₈H₅₄N₇O₈P
• CAS号: 110782-31-5
• 分子量: 887.96
• MDL號碼: MFCD00792622
• 分類程式碼代碼: 41116105
• NACRES: NA.51

属性

• 生物源: non-animal source (no BSE/TSE risk)
• 品質等級: 300
• 產品線: Proligo Reagents
• 化驗: ≥99% (³¹P-NMR); ≥99.0% (redox titration)
• 形狀: powder
• 雜質: ≤0.3% mA2 (reversed phase HPLC, Hydrolysate); ≤0.3% mA3 (reversed phase HPLC, DMT-rA(bz)me); ≤0.3% water content (Karl Fischer); ≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR); ≤0.5% single Impurity (redox titration); ≤1.0% mA1 (reversed phase HPLC, DMT-rA(bz)me-DMT); ≤3% residual Solvent content
• 顏色: white to off-white
• 適合性: conforms to structure for H-NMR; conforms to structure for LC-MS
• 相容性: configured for MerMade
• 儲存溫度: 2-8°C
• SMILES 字串: CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6c(NC(=O)c7ccccc7)ncnc56
• InChI: 1S/C48H54N7O8P/c1-32(2)55(33(3)4)64(61-28-14-27-49)63-42-40(62-47(43(42)59-7)54-31-52-41-44(50-30-51-45(41)54)53-46(56)34-15-10-8-11-16-34)29-60-48(35-17-12-9-13-18-35,36-19-23-38(57-5)24-20-36)37-21-25-39(58-6)26-22-37/h8-13,15-26,30-33,40,42-43,47H,14,28-29H2,1-7H3,(H,50,51,53,56)/t40-,42-,43-,47-,64?/m1/s1
• InChI 密鑰: AZCGOTUYEPXHMJ-PSVHYZMASA-N

说明

一般說明

Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotectionschemes that are based on the application of aliphatic amines, such asmethylamine. The adenosine and guanosine monomers are protected bythe standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analog that is characterized by theexceptional hybridization properties that it imparts with complimentaryDNA or RNA, as well as increased stability against enzymatic degradationcompared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA had foundwidespread use in the fields of:

• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecules

特點和優勢

• High yield of crude oligonucleotides
• Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rA(bz) Phosphoramidite is configured for MerMade Synthesizers.

其他說明

The synthesis cycle for 2′O-Methyloligoribonucleotides consists of the sameseries of reactions as the cycle that is employed for DNA monomers.However, the rate of coupling for 2′O-Methyl RNA monomers is slowercompared to that of DNA monomers (a coupling time of 6 minutes isrecommended for 2′O-Methyl RNA monomers compared to 90 seconds forDNA monomers). With the exception of the 2′O-Methyl RNA monomers andsupports, RNA synthesis is accomplished with the same reagents as DNAsynthesis. All 2′O-Methyl RNA phosphoramidites from Sigma-Aldrich arediluted with dry acetonitrile.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable