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Merck

A0410

Sigma-Aldrich

氨基烯丙基-dUTP 钠盐

≥85%, lyophilized powder

别名:

5-[3-氨基烯丙基]-2′-脱氧尿苷 5′-三磷酸盐, AA-dUTP

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About This Item

经验公式(希尔记法):
C12H20N3O14P3 · xNa+
分子量:
523.22 (free acid basis)
分類程式碼代碼:
41106305
NACRES:
NA.51

品質等級

化驗

≥85%

形狀

lyophilized powder

運輸包裝

dry ice

儲存溫度

−70°C

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應用

氨基烯丙基-dUTP可用于通过聚合酶链式反应(PCR),在第一链cDNA的合成过程中进行间接稳定标记。氨基烯丙基-dUTP可用作反应性位点,用于后续的反应性荧光探针的标记。氨基烯丙基-dUTP也可用于构建反应性DNA支架。
氨基烯丙基-dUTP钠盐已被用于:
  • 添加至脱氧核苷三磷酸混合物中用于互补DNA(cDNA)的合成
  • 生成染色质免疫沉淀(ChIP)探针
  • 在RNA的逆转录中用于合成荧光标记的探针

生化/生理作用

氨基烯丙基-dUTP钠盐/5-(3-氨基烯丙基)-2′-脱氧尿苷5′-三磷酸(aa-dUTP)主要用于合成核酸杂交探针的两步标记法中。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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