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重組細胞
expressed in E. coli
形狀
crystalline
crystals
powder or flakes
比活性
≥10 U/mg
儲存溫度
−20°C
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一般說明
酯酶属于水解酶超家族。该重组酯酶含有一个C末端组氨酸标签。
應用
来自枯草芽孢杆菌的酯酶可用于蛋白质工程研究以及对芳基脂族叔醇乙酸酯的动力学分辨进行研究。产品96667是经重组并大肠杆菌（≥10 U/mg）中表达的。
生化／生理作用
酯酶可参与酯的立体定向水解和生产。从培养的细菌和真菌中获得的酯酶具有多种工业应用。
酯酶是将酯分解为酸和醇的水解酶。
包裝
无底玻璃瓶。内含物在插入的融合锥体内。
單位定義
1U相当于在 pH 7.5，30°C 下每分钟转化1 μmol 4-硝基苯基-L-乙酸盐的酶量。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
High-Resolution Fractionation Processes
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Soil-based gene discovery: a new technology to accelerate and broaden biocatalytic applications
Gray K A, et al.
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New citation. Highly Enantioselective Synthesis of Arylaliphatic Tertiary Alcohols using Mutants of an Esterase from Bacillus subtilis
Robert Kourist, Sebastian Bartsch, et al.
Advanced Synthesis & Catalysis, 349, 1393-1398 (2007)
Birgit Heinze et al.
Protein engineering, design & selection : PEDS, 20(3), 125-131 (2007-02-21)
Enzyme-catalyzed kinetic resolutions of secondary alcohols are a standard procedure today and several lipases and esterases have been described to show high activity and enantioselectivity. In contrast, tertiary alcohols and their esters are accepted only by a few biocatalysts. Only
Jessica Lusty Beech et al.
RSC advances, 12(13), 8119-8130 (2022-04-16)
Esterase enzymes catalyze diverse hydrolysis reactions with important biological, commercial, and biotechnological applications. For the improvement of these biocatalysts, there is a need for widely accessible, inexpensive, and adaptable activity screening assays that identify enzymes with particular substrate specificities. Natural
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