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Merck

61048

Sigma-Aldrich

Abberior® STAR 488, NHS酯

for STED application

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

形狀

solid

濃度

≥50.0% (degree of coupling)

溶解度

DMF: 1 mg/mL, clear

螢光

λex 510 nm; λem 514-534 nm in PBS, pH 7.4

儲存溫度

−20°C

一般說明

Abberior STAR 488是STED显微镜的最新发展。它是一种非常明亮的绿色荧光染料,可以在主要的488 nm激光波长上非常有效地被激发。Abberior STAR 488可以替代Oregon绿488、Atto 488或Alexa Fluor 488等染料。
STED:在STED显微镜中可在585-605 nm处最有效地耗尽染料。

Abberior STAR 488是可在488 nm处激发的染料。并且,该染料专门设计和测试与我们的STAR 440Sx(在590 nm处激发)进行联合使用,用于双色STED显微镜中。该染料已在Leica 双色TCS STED CW显微镜中成功测试。

关键特性
  • 可在488 nm激发的STED染料
  • 在STED显微镜中的理想耗尽行为(〜590 nm)
  • STAR 440SX的双色标记组合,可用于双色STED显微镜

最大吸收波长,λmax: 503 nm (PBS, pH 7.4)
消光系数,ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
校正系数,CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
校正系数 CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
荧光最大值,λfl: 524nm (PBS, pH 7.4)
推荐的STED波长,λSTED: 585 - 605 nm
荧光量子产率,η: 0.89 (PBS, pH 7.4)
荧光寿命,τ: 3.9 ns (MeOH)

應用

抗兔IgG抗体® STAR 488抗体已作为二抗用于:
  • 在前列腺癌细胞系PPC-1和TSU-Pr1中进行荧光免疫组化分析
  • 用于HeLa和猴肾COS-7细胞的免疫荧光中
  • OLN-t40-α-syn细胞(少突胶质细胞)中的STED(刺激发射损耗)显微镜分析

適合性

设计和测试用于荧光超分辨率显微镜

法律資訊

Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH

相關產品

产品编号
说明
价格

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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