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Merck

04529

Sigma-Aldrich

腈水解酶

recombinant, expressed in E. coli, ≥2.0 U/mg

别名:

Nitrile aminohydrolase

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

形狀

crystals
powder

比活性

≥2.0 U/mg

分子量

41 kDa

儲存溫度

2-8°C

一般說明

腈水解酶属于C-N水解酶超家族。它具有催化三联体基序:谷氨酸,赖氨酸和半胱氨酸,分布在真菌,植物和动物中。该蛋白的等电点(pI)为8.1。天然聚丙烯酰胺凝胶电泳(PAGE)。

應用

腈水解酶可用于丙烯酰基交联纤维素二醛(ACCD)的固定化,用于催化活性研究。

生化/生理作用

腈水解酶催化氨的释放和腈形成羧酸。腈水解酶的寡聚结构组织对其功能至关重要。它显示出广泛的底物特异性。

包裝

无底玻璃瓶。内含物装在插入的融合锥内。

單位定義

1U对应于在pH 7.2和25℃下每分钟释放1 μmol氨且丙烯腈转化为丙烯酸的酶的量

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Alena Petříčková et al.
Applied microbiology and biotechnology, 93(4), 1553-1561 (2011-09-06)
Nitrilases from Aspergillus niger CBS 513.88, A. niger K10, Gibberella moniliformis, Neurospora crassa OR74A, and Penicillium marneffei ATCC 18224 were expressed in Escherichia coli BL21-Gold (DE3) after IPTG induction. N. crassa nitrilase exhibited the highest yield of 69,000 U L(-1)
Einar J Stauber et al.
PloS one, 7(4), e35545-e35545 (2012-04-27)
Plants have evolved a variety of mechanisms for dealing with insect herbivory among which chemical defense through secondary metabolites plays a prominent role. Physiological, behavioural and sensorical adaptations to these chemicals provide herbivores with selective advantages allowing them to diversify
Chen-Sheng Zhang et al.
Applied microbiology and biotechnology, 95(1), 91-99 (2012-03-29)
(R)-o-Chloromandelic acid is the key precursor for the synthesis of Clopidogrel®, a best-selling cardiovascular drug. Although nitrilases are often used as an efficient tool in the production of α-hydroxy acids, there is no practical nitrilase specifically developed for (R)-o-chloromandelic acid.
Shivani Jamwal et al.
International journal of biological macromolecules, 131, 117-126 (2019-03-08)
Immobilization of enzymes to improve their catalytic properties is an attractive protocol which makes them suitable candidates to meet various industrial demands. Present study describes the synthesis of new acryloyl crosslinked cellulose dialdehyde (ACCD) for nitrilase immobilization. Nitrilase was immobilized
Joanna E Raczynska et al.
Journal of structural biology, 173(2), 294-302 (2010-11-26)
The nitrilase superfamily is a large and diverse superfamily of enzymes that catalyse the cleavage of various types of carbon-nitrogen bonds using a Cys-Glu-Lys catalytic triad. Thermoactive nitrilase from Pyrococcus abyssi (PaNit) hydrolyses small aliphatic nitriles like fumaro- and malononitryl.

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