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U1250

Sigma-Aldrich

尿素

ReagentPlus®, ≥99.5%, pellets

别名:

碳酰二胺, 碳酰胺

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About This Item

线性分子式:
NH2CONH2
CAS号:
57-13-6
分子量:
60.06
Beilstein:
635724
EC號碼:
200-315-5
MDL號碼:
MFCD00008022
分類程式碼代碼:
12352100
PubChem物質ID:
57654718
NACRES:
NA.21

品質等級

300

產品線

ReagentPlus®

化驗

≥99.5%

形狀

pellets

mp

132-135 °C (lit.)

溶解度

H2O: 8 M

密度

1.335 g/mL at 25 °C (lit.)

SMILES 字串

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI 密鑰

XSQUKJJJFZCRTK-UHFFFAOYSA-N

基因資訊

human ... CA1(759) , CA2(760)
rat ... Ppm1a(24666)

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相关类别

一般說明

尿素是离液剂，用于蛋白质变性。它扰乱了蛋白质的二级、三级和四级结构中的氢键。尿素还可以干扰 DNA 二级结构中存在的氢键。

應用

尿素已用作蛋白质变性剂。
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。

法律資訊

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCK9644
SLCH6516
SLCG4742
SLCD3955
SLCB9837

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Sheehan D.
Physical Biochemistry: Principles and Applications (2013)
Protein carbonyl determination by a rhodamine B hydrazide-based fluorometric assay.
Georgiou C D, et al.
Redox Biology, 17, 236-245 (2018)
Protein and cell wall polysaccharide carbonyl determination by a neutral pH 2, 4-dinitrophenylhydrazine-based photometric assay.
Georgiou C D, et al.
Redox Biology, 17, 128-142 (2018)
Christos D Georgiou et al.
Redox biology, 17, 128-142 (2018-04-24)
A new 2,4-dinitrophenylhydrazine (DNPH)-based photometric assay is developed for the quantification of carbonyls in protein samples from any biological source by protein carbonyl-DNPH hydrazone formation at acidic pH in the presence of denaturing urea, and subsequent hydrazone solubilization in the
Gurudayal et al.
Nano letters, 15(6), 3833-3839 (2015-05-06)
Photoelectrochemical water splitting half reactions on semiconducting photoelectrodes have received much attention but efficient overall water splitting driven by a single photoelectrode has remained elusive due to stringent electronic and thermodynamic property requirements. Utilizing a tandem configuration wherein the total
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