PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• 别名: 1X Bis-Tris transfer buffer, Bis-Tris
• 分類程式碼代碼: 41105319
• NACRES: NA.25

属性

• 產品線: pHast Pack^™
• 品質等級: 100
• 形狀: powder
• 儲存條件: (Tightly closed. Dry)
• pH值: 7.2
• 正離子痕跡: Pb: ≤5 ppm
• 異物活動: DNAse, free; NICKase, free; Protease, free; RNAse, free
• 儲存溫度: room temp

说明

一般說明

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

應用

Bis-Tris buffer may be used in western blot as a transfer buffer.

特點和優勢

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

包裝

Foil pouches

重構

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

儲存和穩定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他說明

For additional information on our range of Biochemicals, please complete this form.

法律資訊

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全信息

• 象形圖: GHS05
• 訊號詞: Danger
• 危險聲明: H318
• 防範說明: P280 - P305 + P351 + P338
• 危險分類: Eye Dam. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3