02538

Raka-Ray 琼脂

suitable for microbiology, NutriSelect^® Plus

• 别名: 乳酸菌选择性琼脂基础
• 分類程式碼代碼: 41171609
• NACRES: NA.85

属性

• 無菌: non-sterile
• 品質等級: 200
• 形狀: powder
• 儲存期限: limited shelf life, expiry date on the label
• 成份: N-acetyl glucosamine, 0.5 g/L ; agar, 17.0 g/L ; betaine hydrochloride, 2.0 g/L ; casein enzymic hydrolysate, 20.0 g/L ; diammonium hydrogen-citrate, 2.0 g/L ; fructose, 5.0 g/L ; glucose, 5.0 g/L ; liver concentrate, 1.0 g/L ; magnesium sulphate, 2.0 g/L ; maltose, 10.0 g/L ; manganese sulfate, 0.66 g/L ; monopotassium phosphate, 2.0 g/L ; potassium aspartate, 2.5 g/L ; potassium glutamate, 2.5 g/L ; yeast extract, 5.0 g/L
• 製造商／商標名: NutriSelect^® Plus
• 最終pH值: 5.4±0.2 (25 °C)
• 應用: agriculture; bioburden testing; food and beverages; microbiology
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 適合性: Pediococcus spp.; selective for Lactobacillus spp.

说明

一般說明

Raka-Ray Agar was formulated by Saha, Sondag and Middlekauff to monitor the brewing process and analyze it for a wide range of bacteria. The media is found to be superior to several other media for the cultivation of Lactobacilli and Pediococci. It also suppresses the growth of non-lactic acid facultative bacteria that are often associated with lactic beer spoilage.

應用

Raka-Ray Agar is recommended for selective isolation of lactic acid bacteria encountered in beer and the brewing process.

準備報告

Suspend 38.5 g in 500 ml of distilled water. Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and add contents of 1vial of lactic acid bacteria selective supplement (Cat. No. 14121). Add 3.5 ml of Cycloheximide solution (Cat. No. 18079). Mix well and pour into sterile petri dishes or dispense as desired.

分析報告

Casein enzymic hydrolysate, liver concentrate, yeast extract provide carbon, nitrogen, amino acids, minerals, vitamins, trace elements and other essential nutrients for growth. Potassium aspartate and potassium glutamate are additional sources of amino acids. Maltose is to detect lactobacilli, which cannot utilize glucose as a carbon source and fructose is the carbon source of Lactobacillus fructivorans. Glucose is needed as the fermentative energy source for the Pediococci. Sorbitan mono-oleate act as a stimulant for lactic acid bacteria in general. Also, N-acetyl glucosamine, liver concentrate yeast extract and betaine hydrochloride are used as growth stimulating agents. Diammonium hydrogen-citrate and Monopotassium phosphate are the buffering agents while Magnesium and Manganese are important trace elements for Lactobacillaceae. The addition of phenylethanol inhibits Gram-negative organisms, while amphotericin B and cycloheximide inhibits growth of yeasts and molds.

腳註

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律資訊

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H319
• 防範說明: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危險分類: Eye Irrit. 2
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)