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形狀
solution
分子量
239.5
包裝
pkg of 2 × 25 mL (1 g)
製造商／商標名
Roche
濃度
0.1-1.0 mg/mL
技術
electrophoresis: suitable
mp
175-185 °C
溶解度
water: soluble 0.1 M
儲存溫度
2-8°C
一般說明
Set consists of Pefabloc® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc® SC protects proteins throughout procedures such as:
Use Pefabloc® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.
- Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi)
- Subsequent purification steps
- Varied sample storage conditions
- Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.)
- Biochemical studies where proteins are required.
Use Pefabloc® SC to inactivate proteinase K, for example, during pulsed-field gel electrophoresis (PFGE). With this technique, isolating the genomic DNA requires proteinase K to degrade cellular components, and this highly resilient protease is difficult to inactivate. Pefabloc® SC inhibits proteinase K, and protects the stability of restriction enzymes used for further DNA analysis.
The Pefabloc® SC PLUS set combines the serine protease inhibitor Pefabloc® SC with a uniquely formulated Pefabloc® SC protector (PSC-Protector), adding additional convenience and reliability to the safe, stable protease inhibition provided by Pefabloc® SC .
Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc® SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.
Recent findings indicate that sulfonyl-type serine protease inhibitors such as Pefabloc® SC and PMSF can bind covalently to proteins. This can occur when the inhibitors are used in high concentrations, or during extended incubation times under alkaline conditions. This interaction adversely affects the tyrosine and lysine residues of a protein, as well as the free amino terminus. The PLUS additive (PSC-Protector) included with this set prevents covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH.
特異性
Specific, potent, and irreversible inhibitor of serine proteases. The inhibitory activity of Pefabloc® SC is comparable to PMSF or DFP; however, Pefabloc® SC is non-toxic. Specifically inhibits serine proteases and prevents nonspecific covalent modification of proteins as tested by mass spectrometry.
特點和優勢
- Take advantage of a simplified two-reagent system. Use the PSC-Protector solution to prevent covalent binding between proteins and Pefabloc® SC, even at high concentrations, extended incubation times, and at alkaline pH (see Figure 1 and Figure 2).
- Benefit from an easy-to-use inhibitor. Add water-soluble Pefabloc® SC directly to aqueous buffers.
- Avoid hazardous compounds. Obtain non-toxic protease inhibition without risk to you, or those around you.
- Ensure protection with improved stability. Achieve consistent protease inhibition even at pH levels above 7.0 and temperatures above +4°C.
- Maximize inhibition. Be certain that your levels of active inhibitor are high enough by using a product that is reliably soluble and stable.
包裝
Set consists of Pefabloc® SC and the corresponding amount of PLUS additive to avoid covalent attachment of Pefabloc® SC to proteins when used in higher concentrations and extended incubation times at an alkaline pH. Choose Pefabloc® SC for all applications where the general inhibition of serine proteases is desired. With its high stability and irreversible inhibition mechanism, Pefabloc® SC protects proteins throughout procedures such as:
- Extraction processes (from animal tissues or cells, plants, bacteria, yeast, and fungi)
- Subsequent purification steps
- Varied sample storage conditions
- Downstream protein detection (e.g., western blotting, reporter gene analysis, etc.)
- Biochemical studies where proteins are required.
Set containing two components
其他說明
仅用于生命科学研究。不可用于诊断。
法律資訊
Pefabloc is a registered trademark of Pentapharm
仅试剂盒组分
产品编号
说明
- Pefabloc SC, 1 g
- PSC-Protector solution, 2 x 25 mL ready-to-use
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1B
儲存類別代碼
3 - Flammable liquids
水污染物質分類（WGK）
WGK 1
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