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化驗
97.7% (HPLC)
品質等級
形狀
solution
分子量
Mr 987.5 (biotin-16-UTP-Li4)
包裝
pkg of 25 μL (250 nmol; 10mM)
製造商/商標名
Roche
顏色
colorless
溶解度
water: miscible
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
生物素-16-UTP中的生物素通过酰胺键与尿苷三磷酸结合。生物素-16-UTP是SP6、T3和T7 RNA聚合酶的底物。它可以取代体外转录反应中的UTP,用于RNA标记。利用ATP、GTP、CTP、UTP和生物素-16-UTP和相应的RNA聚合酶分别对具有T7、SP6或T3启动子的线性模板DNA进行体外转录。
應用
生物素标记的RNA可作为杂交探针用于:
- Northern 印迹
- Southern 印迹
- 菌落/菌斑转移筛选
- RNase保护实验
- 原位杂交
- 芯片杂交
品質
典型分析:至少85%生物素-16-UTP(HPLC,面积%)。
外觀
生物素-16-UTP,四锂盐,10mM溶液
其他說明
仅用于生命科学研究。不用于诊断操作。
水污染物質分類(WGK)
WGK 1
閃點(°F)
does not flash
閃點(°C)
does not flash
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