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Merck

11388908910

Roche

生物素-16-UTP

pkg of 25 μL (250 nmol; 10mM)

别名:

生物素-16-UTP, 生物素

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About This Item

分類程式碼代碼:
41116100

化驗

97.7% (HPLC)

品質等級

形狀

solution

分子量

Mr 987.5 (biotin-16-UTP-Li4)

包裝

pkg of 25 μL (250 nmol; 10mM)

製造商/商標名

Roche

顏色

colorless

溶解度

water: miscible

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

生物素-16-UTP中的生物素通过酰胺键与尿苷三磷酸结合。生物素-16-UTP是SP6、T3和T7 RNA聚合酶的底物。它可以取代体外转录反应中的UTP,用于RNA标记。利用ATP、GTP、CTP、UTP和生物素-16-UTP和相应的RNA聚合酶分别对具有T7、SP6或T3启动子的线性模板DNA进行体外转录。

應用

生物素标记的RNA可作为杂交探针用于:
  • Northern 印迹
  • Southern 印迹
  • 菌落/菌斑转移筛选
  • RNase保护实验
  • 原位杂交
  • 芯片杂交
随后,使用荧光链霉亲和素结合物检测标记的RNA,或使用链霉亲和素-AP结合物通过酶联免疫吸附测定(ELISA)检测标记的RNA。

品質

典型分析:至少85%生物素-16-UTP(HPLC,面积%)。

外觀

生物素-16-UTP,四锂盐,10mM溶液

其他說明

仅用于生命科学研究。不用于诊断操作。

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


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Vascular smooth muscle cell (VSMC) proliferation causes intimal thickening in atherosclerosis and restenosis. Previously, we demonstrated that Wnt/β-catenin signaling upregulates VSMC proliferation in vitro. We examined this pathway in vivo and investigated the involvement of specific Wnt proteins in VSMC

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