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Merck
所有图片(1)

主要文件

10901393001

Roche

糖原

from mussels

别名:

糖原

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About This Item

分類程式碼代碼:
41116100

品質等級

形狀

solution

包裝

pkg of 1 mL (20 mg)

製造商/商標名

Roche

儲存溫度

−20°C

應用

该制剂可用作核酸(DNA或RNA)沉淀的载体。作为惰性材料,它可以代替tRNA或超声处理的DNA。
20 μg糖原(1 μl溶液)可以从1 ml的体积中沉淀出pg量的DNA或RNA。
在一个典型实验中,将5 pg [3H]标记的小牛胸腺DNA溶解于含1 mM EDTA、0.4 M LiCl的500 μl 10 mM Tris-HCl(pH 8.0)中。加入1 μl糖原溶液(20 μg糖原)作为载体,然后在-15至-25 °C下用1.2 ml乙醇沉淀,并在-15至-25 °C下保存3小时。 离心(12 000×g 10分钟)后,总放射性存在于沉淀物中。不添加糖原,DNA不会沉淀。

特點和優勢

糖原具有特殊的分子生物学性质,是核酸制剂中的惰性载体。

内容
水溶液,20 mg/ml

品質

根据现行质量控制程序,检测是否存在核酸内切酶、切割活性、核酸外切酶、核糖核酸酶、核酸和蛋白酶。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

does not flash

閃點(°C)

does not flash


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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With the exception of lamina-associated domains, the radial organization of chromatin in mammalian cells remains largely unexplored. Here we describe genomic loci positioning by sequencing (GPSeq), a genome-wide method for inferring distances to the nuclear lamina all along the nuclear
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