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描述
Molecular Formula: C33H39N8O6COOCH3
品質等級
化驗
90% (enzymatic)
形狀
powder
分子量
Mr 702.9
包裝
pkg of 20 mg
製造商／商標名
Roche
儲存溫度
room temp
一般說明
Chromozym® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.
應用
Use Chromozym® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.
品質
Contaminant: <0.5% free 4-nitraniline
序列
Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate
原則
Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.
準備報告
Activator: Mg2+ (Km = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn2+, Fe2+, Zn2+)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.
其他說明
仅用于生命科学研究。不可用于诊断。
法律資訊
Chromozym is a registered trademark of Pentapharm AG, Basel
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
R Ito et al.
Clinical chemistry, 27(4), 586-593 (1981-04-01)
The amidolytic activity of activated kallikrein in plasma can be measured by use of the chromogenic substrate, S-2302 (H-D-Pro-Phe-Arg-pNA). Plasma prekallikrein was activated to kallikrein by exposure to 50 mg/L dextran sulfate in acetone/water (35/65 by vol) at 0 degrees
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