SCT132

BioTracker 510 Green C2(FM2-10) Synaptic Dye

Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.

• 别名: Live cell imaging probe
• 分類程式碼代碼: 12352207
• NACRES: NA.47

属性

• 技術: cell based assay: suitable
• 檢測方法: fluorometric
• 運輸包裝: ambient

说明

一般說明

BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.

BioTracker 510 Green C2(FM2-10) Synaptic Dye is more water soluble than BioTracker 510 Green C4(FM1-43) Synaptic Dye and thus has a faster de-staining rate.

Spectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm

Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nm

應用

Live cell fluorescent imaging

Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.

Research Category
Cell Imaging

Research Sub Category
Live Cell Dye

品質

Spectral Properties
Absorbance (MeOH): 510 nm
Emission (MeOH): 625 nm

Absorbance (Membranes): 480 nm
Emission (Membranes): 600 nm

外觀

Lyophilized

儲存和穩定性

Store BioTracker 510 Green C2(FM2-10) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable