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Key Documents

SCR022

Sigma-Aldrich

Adult Rat Hippocampal Neural Stem Cells

Adult Rat Hippocampal Neural Stem Cells are self-renewing cells with the capacity to differentiate into neurons, astrocytes & oligodendrocytes.

别名:

Rat Neural Stem Cells

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About This Item

分類程式碼代碼:
41106509
eCl@ss:
32011203
NACRES:
NA.81

生物源

rat

品質等級

製造商/商標名

Chemicon®

技術

cell culture | stem cell: suitable

輸入

sample type neural stem cell(s)

運輸包裝

liquid nitrogen

一般說明

Adult Rat Hippocampal Neural Stem Cells

Introduction:

Neural stem cells (NSC) are self-renewing cells with the capacity to differentiate into neurons, astrocytes and oligodendrocytes. Adult NSC are extremely rare and are believed to reside only in discrete regions of the hippocampus and the subventricular zone. Due to their therapeutic promise, considerable attention has been focused on identifying the sources of stem cells, the signals that regulate their proliferation and the specification of neural stem cells towards more differentiated cell lineages (Gage, 2000).

Presently, neural stem cells are often identified based upon the presence of molecular markers that are correlated with the stem and/or progenitor state along with the absence of differentiated phenotypes as assessed through marker analysis. To aid researchers in the accurate identification and characterization of neural stem cells, Millipore presents cryopreserved Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCR022).

Millipore′s Cryopreserved Adult Rat Hippocampal Neural Stem Cells provides ready-to use primary neural stem cells isolated from the hippocampus of adult Fisher 344 rats. These primary cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.

We recommend that Millipore′s Cryopreserved Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCR022) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population. Please look for these differentiation assays from CHEMICON in the near future.

For Research Use Only; not for use in diagnostic procedure.

細胞系描述

Neural Stem Cells

應用

Research Category
Stem Cell Research

包裝

1x10(e6) cells

成分

1.1x10e6 viable Adult Rat Hippocampal Neural Stem Cells: (Part No. 2003769) derived from adult female Fisher 344 rats, aged 43-55 days, cryopreserved. Store in liquid nitrogen.

儲存和穩定性

When stored at the recommended storage conditions (liquid nitrogen), adult hippocampal neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.

法律資訊

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

212.0 °F

閃點(°C)

100 °C


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Sehee Kim et al.
International journal of molecular sciences, 18(9) (2017-09-12)
Palmitate concentrations in type 2 diabetic patients are higher than in healthy subjects. The prolonged elevation of plasma palmitate levels induces oxidative stress and mitochondrial dysfunction in neuronal cells. In this study, we examined the role of mdivi-1, a selective
Hyunju Chung et al.
The Journal of endocrinology, 230(2), 239-250 (2016-06-22)
We have previously demonstrated that ghrelin stimulates the cellular proliferation of cultured adult rat hippocampal neural stem cells (NSCs). However, little is known about the molecular mechanisms by which ghrelin regulates cell cycle progression. The purpose of this study was
Elizabeth D Kirby et al.
eLife, 2, e00362-e00362 (2013-04-20)
Stress is a potent modulator of the mammalian brain. The highly conserved stress hormone response influences many brain regions, particularly the hippocampus, a region important for memory function. The effect of acute stress on the unique population of adult neural
Sehee Kim et al.
Journal of molecular endocrinology, 57(3), 171-184 (2016-08-18)
Adult hippocampal neurogenesis is decreased in type 2 diabetes, and this impairment appears to be important in cognitive dysfunction. Previous studies suggest that ghrelin gene products (acylated ghrelin (AG), unacylated ghrelin (UAG) and obestatin (OB)) promote neurogenesis. Therefore, we hypothesize
Seong Gak Jeon et al.
Molecules and cells, 39(8), 603-610 (2016-07-20)
Recently, an increasing number of studies have focused on the effects of CD4+ T cell on cognitive function. However, the changes of Th2 cytokines in restricted CD4+ T cell receptor (TCR) repertoire model and their effects on the adult hippocampal

商品

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

实验方案

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

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