推荐产品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
lyophilized
包含
≤0.1% sodium azide as preservative
物種活性
baboon, monkey, rat, human
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG
運輸包裝
ambient
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... CALB1(793)
rhesus monkey ... Calb1(677721)
一般說明
兔多克隆抗体以冻干未稀释的血清形式提供。可识别钙结合蛋白D-28K蛋白。
兔多克隆抗钙结合蛋白D-28K（Ab-1）可重新识别大鼠海马中的钙结合蛋白D-28K蛋白。经验证可用于冰冻切片和T细胞共刺激。
可识别大鼠海马组织中的钙结合蛋白D-28K蛋白。
免疫原
牛
纯化牛小脑钙结合蛋白D-28K蛋白
應用
冷冻切片（整个安装/振动切片机切片，1:1000，荧光）
振动切片机切片（生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000，请参阅应用参考文献）
振动切片机切片（生物素-链霉亲和素/过氧化物酶检测为1:5000-1:10,000，请参阅应用参考文献）
警告
毒性：标准处理（A）
外觀
未稀释的血清。
重構
用100 µl无菌蒸馏H₂O复溶冻干抗体。所得复溶溶液将包含≤0.1%叠氮化钠。注意复溶小瓶的全部内容物；在运输和处理过程中，冻干颗粒的部分可能已脱落，可能不在小瓶底部。用无菌PBS或Tris缓冲液稀释，稀释度不高于1:10。溶解后，等分并冷冻保存（-20°C）。储备溶液在-20°C下可稳定保存至多6个月。避免溶液反复冻融。
分析報告
阳性对照
大鼠纹状体、海马或皮质
大鼠纹状体、海马或皮质
其他說明
Conde, F., et al. 1994.J. Comp.Neurol.341, 95.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
Heizmann, C.W.1993.Acta Neurobiol.Exp.53, 15.
Baimbridge, K.G., et al. 1992.Trends Neurosci.15, 303.
Heizmann, C.W. and Hunziker, W. 1992.Trends Biochem.Sci.16, 98.
在大鼠纹状体，皮层和海马中检查了抗体特异性。根据生产商说明，使用生物素-链霉亲和素/过氧化物酶试剂检测一抗。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Ana I Arroyo et al.
EMBO molecular medicine, 6(3), 398-413 (2014-01-23)
Understanding the role of lipids in synapses and the aberrant molecular mechanisms causing the cognitive deficits that characterize most lipidosis is necessary to develop therapies for these diseases. Here we describe sphingomyelin (SM) as a key modulator of the dendritic
Daniela Herrera Moro Chao et al.
PloS one, 10(9), e0138107-e0138107 (2015-09-30)
Gaucher disease is characterized by lysosomal accumulation of glucosylceramide due to deficient activity of lysosomal glucocerebrosidase (GBA). In cells, glucosylceramide is also degraded outside lysosomes by the enzyme glucosylceramidase 2 (GBA2) of which inherited deficiency is associated with ataxias. The
Yuko Sugita et al.
Frontiers in neuroscience, 14, 586013-586013 (2020-12-19)
The decline in visual function due to normal aging impacts various aspects of our daily lives. Previous reports suggest that the aging retina exhibits mislocalization of photoreceptor terminals and reduced amplitudes of scotopic and photopic electroretinogram (ERG) responses in mice.
André R A Marques et al.
PloS one, 10(8), e0135889-e0135889 (2015-08-15)
The enzyme glucocerebrosidase (GBA) hydrolyses glucosylceramide (GlcCer) in lysosomes. Markedly reduced GBA activity is associated with severe manifestations of Gaucher disease including neurological involvement. Mutations in the GBA gene have recently also been identified as major genetic risk factor for
Patrick W Keeley et al.
Visual neuroscience, 34, E002-E002 (2017-01-10)
Retinal bipolar cells spread their dendritic arbors to tile the retinal surface, extending them to the tips of the dendritic fields of their homotypic neighbors, minimizing dendritic overlap. Such uniform nonredundant dendritic coverage of these populations would suggest a degree
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