推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
2B, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
同型
IgG2b
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... BRCA2(675)
一般說明
可识别表达野生型BRCA2基因的细胞中的~460 kDa BRCA2蛋白。根据美国专利号5,753,441和6,162,897的许可证销售。
抗BRCA2（Ab-1），小鼠单克隆，克隆2B，可识别表达野生型BRCA2基因的细胞中的~460 kDa BRCA2蛋白。它已被验证可用于蛋白质印迹和免疫沉淀。
通过用指定的免疫原免疫小鼠并将脾细胞与骨髓瘤细胞融合而产生的纯化小鼠单克隆抗体。可识别~460 kDa BRCA2蛋白。
免疫原
人
由人BRCA2的氨基酸1651-1821与GST融合而成的重组蛋白
應用
免疫印迹法（2 µg/ml）
免疫沉淀（2 µg）
免疫沉淀（2 µg）
包裝
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外觀
溶于PBS。
重構
初次融化后，等分并冷冻（-20°C）。
分析報告
阳性对照
MCF7细胞
MCF7细胞
其他說明
Andres, J.L., et al. 1998.Oncogene16, 2229.
Chen, J., et al. 1998.Mol.Cell2, 317.
Chen, P.L., et al. 1998.Proc.Natl.Acad.Sci. USA95, 5287.
Marmorstein, L.Y., et al. 1998.Proc.Natl.Acad.Sci. USA95, 13869.
Connor, F., et al. 1997.Nat. Genet.17, 423.
Sharan, S.K., et al. 1997.Nature386, 804.
Ludwig, T., et al. 1997.Genes Dev.11, 1226.
Mizuta, R., et al. 1997.Proc.Natl.Acad.Sci. USA94, 6927.
Rajan, J.V., et al. 1997.Dev.Biol.184, 385.
Suzuki, A., et al. 1997.Genes Dev.11, 1242.
Wong, A.K.C., et al. 1997.J. Biol. Chem.272, 31941.
Cannon-Albright, L.A. and M.H.Skolnick.1996.Semin.Oncol.23, 1.
Goggins, M., et al. 1996.Cancer Res.56, 5360.
Stratton, M.R.1996.Hum.Mol.Genet.5, 1515.
Tavtigian, S.V., et al. 1996.Nat. Genet.12, 333.
Rajan, J.V., et al. 1996.Proc.Natl.Acad.Sci. USA93, 13078.
Spillman, M.A. and A.M. Bowcock.1996.Oncogene13, 1639.
Vaughn, J.P., et al. 1996.Cancer Res.56, 4590.
Wooster, R., et al. 1995.Nature378, 789.
Chen, J., et al. 1998.Mol.Cell2, 317.
Chen, P.L., et al. 1998.Proc.Natl.Acad.Sci. USA95, 5287.
Marmorstein, L.Y., et al. 1998.Proc.Natl.Acad.Sci. USA95, 13869.
Connor, F., et al. 1997.Nat. Genet.17, 423.
Sharan, S.K., et al. 1997.Nature386, 804.
Ludwig, T., et al. 1997.Genes Dev.11, 1226.
Mizuta, R., et al. 1997.Proc.Natl.Acad.Sci. USA94, 6927.
Rajan, J.V., et al. 1997.Dev.Biol.184, 385.
Suzuki, A., et al. 1997.Genes Dev.11, 1242.
Wong, A.K.C., et al. 1997.J. Biol. Chem.272, 31941.
Cannon-Albright, L.A. and M.H.Skolnick.1996.Semin.Oncol.23, 1.
Goggins, M., et al. 1996.Cancer Res.56, 5360.
Stratton, M.R.1996.Hum.Mol.Genet.5, 1515.
Tavtigian, S.V., et al. 1996.Nat. Genet.12, 333.
Rajan, J.V., et al. 1996.Proc.Natl.Acad.Sci. USA93, 13078.
Spillman, M.A. and A.M. Bowcock.1996.Oncogene13, 1639.
Vaughn, J.P., et al. 1996.Cancer Res.56, 4590.
Wooster, R., et al. 1995.Nature378, 789.
在单个BRCA2等位基因中具有6174delT突变的CAPAN-1细胞系表达230 kDa的截短的BRCA2蛋白，该蛋白包含抗BRCA2表位并保留RAD51结合但失去了p53结合。全长BRCA2蛋白将在SDS-PAGE上与DNA PK的催化亚基共迁移，可以使用抗DNA PK（目录编号PC127）检测。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律資訊
根据美国专利号5,837,492和6,124,104的许可证销售。
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
M Goggins et al.
Cancer research, 56(23), 5360-5364 (1996-12-01)
Germline mutations in BRCA2 predispose carriers to the development of breast, ovarian, and a variety of other cancers. The original localization of the BRCA2 gene was aided by its homozygous deletion in a pancreatic carcinoma; indeed, an excess of pancreatic
A K Wong et al.
The Journal of biological chemistry, 272(51), 31941-31944 (1998-01-24)
Recent work has shown that the murine BRCA2 tumor suppressor protein interacts with the murine RAD51 protein. This interaction suggests that BRCA2 participates in DNA repair. Residues 3196-3232 of the murine BRCA2 protein were shown to be involved in this
F Connor et al.
Nature genetics, 17(4), 423-430 (1997-12-17)
Germline mutation of the BRCA2 gene carries a high risk of developing breast cancer. To study the function of this gene, we generated a mutation in Brca2 in mice. Unlike other mutations in the Brca2 gene, which are lethal early
J V Rajan et al.
Developmental biology, 184(2), 385-401 (1997-04-15)
Germline mutations in the putative tumor suppressor gene, BRCA1, predispose women to dramatically elevated risks of breast cancer, while germline mutations in the structurally unrelated gene, BRCA2, predispose both men and women to breast cancer. Recent studies have suggested an
S V Tavtigian et al.
Nature genetics, 12(3), 333-337 (1996-03-01)
Breast carcinoma is the most common malignancy among women in developed countries. Because family history remains the strongest single predictor of breast cancer risk, attention has focused on the role of highly penetrant, dominantly inherited genes in cancer-prone kindreds (1).
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