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生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
K36/15, monoclonal
物種活性
mouse, rat
技術
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG2bκ
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
rat ... Kcna1(24520)
一般說明
Potassium voltage-gated channel subfamily A member 1, better known as Potassium Channel Kv1.1, or Kcna1 belongs to the Shaker potassium channel group responsible for mediating ion permeability of excitable membranes. Conformational changes as a result of voltage changes across the cell membrane varies the permeability of this multi-pass transmembrane channel to potassium. Mutations of the Shaker potassium channel gene in Drosophila and the KCNA1 gene for its mammalian orthologue, Kv1.1, reduce the probability of action potential failure thereby causing hyperexcitability near axon branch points and nerve terminals, thereby leading to uncontrolled movements and recapitulating the episodic ataxia-1 (EA1) symptoms in human patients. Further, the axonal Kv1 channel activity may regulate synaptic transmission by modulating the action potential waveform, duration, firing pattern, and spread of depolarization into axonal branches.
特異性
Other homologies: Human (94% sequence homology).
免疫原
Linear peptide corresponding to 18 amino acids from the extracellular domain of rat Potassium Channel Kv1.1.
應用
Anti-Potassium Channel Kv1.1 Antibody, clone K36/15 is a highly specific mouse monoclonal antibody, that targets Kv1.1 & has been tested in Western blotting, Immunofluorescence & IHC.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western Blotting Analysis: 4 µg/mL from a representative lot detected Potassium Channel Kv1.1 in 10 µg of rat brain membrane tissue lysate.
Immunofluoresense Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Tiffany, A. M., et al. (2000). J Cell Biol. 148(1):147-158.).
Immunhistochemistry Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Wenzel. H. J., et al (2007). Epilepsia. 48(11):2023-2046.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detect Potassium Channel Kv1.1 in wild type mouse hippocampus tissue, and demonstrated a loss of signal in Potassium Channel Kv1.2 knock out mouse hippocampus tissue (Prof. J. Trimmer, University of California, Davis.).
Immunofluoresense Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Tiffany, A. M., et al. (2000). J Cell Biol. 148(1):147-158.).
Immunhistochemistry Analysis: A representative lot from an independent laboratory detected Potassium Channel Kv1.1 in Kv1.1 transfected COS-1 cells (Wenzel. H. J., et al (2007). Epilepsia. 48(11):2023-2046.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detect Potassium Channel Kv1.1 in wild type mouse hippocampus tissue, and demonstrated a loss of signal in Potassium Channel Kv1.2 knock out mouse hippocampus tissue (Prof. J. Trimmer, University of California, Davis.).
品質
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Potassium Channel Kv1.1 in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Potassium Channel Kv1.1 in 10 µg of mouse brain tissue lysate.
標靶描述
~65 kDa observed. The calculated molecular weight of this protein is 56 kDa However, it can run higher due to glycosylation (Deal, K. K., et al. (1994). J Neurosci. 14(3 Pt 2):1666-1676.).
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Voltage-gated potassium channels and the diversity of electrical signalling.
Jan, Lily Yeh and Jan, Yuh Nung
The Journal of Physiology, 590, 2591-2599 (2012)
A M Tiffany et al.
The Journal of cell biology, 148(1), 147-158 (2000-01-12)
Mechanisms of ion channel clustering by cytoplasmic membrane-associated guanylate kinases such as postsynaptic density 95 (PSD-95) and synapse-associated protein 97 (SAP97) are poorly understood. Here, we investigated the interaction of PSD-95 and SAP97 with voltage-gated or Kv K(+) channels. Using
Axon physiology.
Debanne, Dominique, et al.
Physiological Reviews, 91, 555-602 (2011)
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